4-HNE (4-Hydroxynonenal) ELISA Kit
- SKU:
- AEES00007
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Size:
- 96 Assays
- Sensitivity:
- 0.38 ng/mL
- Range:
- 0.63-40 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
| Product Name: | 4-HNE (4-Hydroxynonenal) ELISA Kit |
| Product Code: | AEES00007 |
| Assay Type: | Competitive |
| Format: | 96T |
| Assay Time: | 2.0h |
| Detection Range: | 0.63-40 ng/mL |
| Sensitivity: | 0.38 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 50μL |
| Specificity: | This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50μL Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
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