Metabolism Assays

Alcohol dehydrogenase Activity Assay Kit (BA0011)

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SKU:
BA0011
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Biological (Plasma, Serum, Urine, Tissue, Culture Media)
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry

Description

ELISA Kit Technical ManualMSDS

Alcohol dehydrogenase Activity Assay Kit - Information

Assay Genie's non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Applications

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

Alcohol dehydrogenase Activity Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Alcohol dehydrogenase Activity Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 10 mL Diaphorase : 120 uL NAD Solution: 1 mL Calibrator: 1.5 mL MTT Solution: 1.5 mL Substrate: 1 mL 10% Ethanol
Kit RequiresPipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader.
Method of DetectionOD565nm
Detection Limit0.4 U/L
SamplesBiological (e.g. plasma, serum, urine, tissue and culture media)
SpeciesAll
Protocol Length30 min
Size100 tests
StorageStore all components at -20°C upon receiving
Shelf Life6 months

More Details

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.
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