Name: Anti-ABHD5 Antibody (CAB7592)
Brand: Developmental Biology
SKU: CAB7592
Availability: OutOfStock

Antibody Name:	Anti-ABHD5 Antibody
Antibody SKU:	CAB7592
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human ABHD5 (NP_057090.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Mouse testis, Mouse lung, Rat spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human ABHD5 (NP_057090.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAE EEEV DSAD TGER SGWL TGWL PTWC PTSI SHLK EAEE KMLK CVPC TYKK EPVR ISNG NKIW TLKF SHNI SNKT PLVL LHGF GGGL GLWA LNFG DLCT NRPV YAFD LLGF GRSS RPRF DSDA EEVE NQFV ESIE EWRC ALGL DKMI LLGH NLGG FLAA AYSL KYPS RVNH LILV EPWG FPER PDLA DQDR PIPV WIRA LGAA LTPF NPLA GLRI AGPF GLSL VQRL RPDF KRKY SSMF EDDT VTEY IYHC NVQT PSGE TAFK NMTI PYGW AKRP MLQR IGKM HPDI PVSV IFGA RSCI DGNS GTSI QSLR PHSY VKTI AILG AGHY VYAD QPEE FNQK VKEI CDTV D
Gene ID:	51099
Uniprot:	Q8WTS1
Cellular Location:	Cytoplasm, Lipid droplet
Calculated MW:	39kDa
Observed MW:	39kDa

Synonyms:

ABHD5, CDS, CGI58, IECN2, NCIE2

Background:

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

UniProt Protein Function:	ABHD5: a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Colocalized with PLIN and ADFP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues, and Chanarin-Dorfman syndrome (CDS), a triglyceride storage disease with impaired long-chain fatty acid oxidation and icthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons.
UniProt Protein Details:	Protein type:Transferase; EC 2.3.1.51; Lipase Chromosomal Location of Human Ortholog: 3p21 Cellular Component: intracellular membrane-bound organelle; cytoplasm; lipid particle; nucleus; cytosol Molecular Function:triacylglycerol lipase activity; 1-acylglycerol-3-phosphate O-acyltransferase activity; lysophosphatidic acid acyltransferase activity Biological Process: triacylglycerol catabolic process; phosphatidic acid biosynthetic process; positive regulation of lipoprotein lipase activity; fatty acid metabolic process; cell differentiation Disease: Chanarin-dorfman Syndrome
NCBI Summary:	The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WTS1
NCBI GenInfo Identifier:	73921640
NCBI Gene ID:	51099
NCBI Accession:	Q8WTS1.1
UniProt Secondary Accession:	Q8WTS1,Q9Y369, B2R9K0,
UniProt Related Accession:	Q8WTS1
Molecular Weight:
NCBI Full Name:	1-acylglycerol-3-phosphate O-acyltransferase ABHD5
NCBI Synonym Full Names:	abhydrolase domain containing 5
NCBI Official Symbol:	ABHD5
NCBI Official Synonym Symbols:	CDS; CGI58; IECN2; NCIE2
NCBI Protein Information:	1-acylglycerol-3-phosphate O-acyltransferase ABHD5; lipid droplet-binding protein CGI-58; abhydrolase domain-containing protein 5
UniProt Protein Name:	1-acylglycerol-3-phosphate O-acyltransferase ABHD5
UniProt Synonym Protein Names:	Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58
Protein Family:	1-acylglycerol-3-phosphate O-acyltransferase
UniProt Gene Name:	ABHD5
UniProt Entry Name:	ABHD5_HUMAN

Western blot analysis of extracts of various cell lines, using ABHD5 antibody (CAB7592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-ABHD5 Antibody (CAB6801)

ABHD5 Antibody (PACO07532)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)