Autophagy Antibodies
Anti-ACBD5 Antibody (CAB10595)
- SKU:
- CAB10595
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-ACBD5 Antibody |
| Antibody SKU: | CAB10595 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human ACBD5 (NP_663736.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, LO2, Jurkat, A-549, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human ACBD5 (NP_663736.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TSTP NAKT VNGK AESS DSGA ESEE EEAQ EEVK GAEQ SDND KKMM KKSA DHKN LEVI VTNG YDKD GFVQ DIQN DIHA SSSL NGRS TEEV KPID ENLG QTGK SAVC IHQD INDD HVED VTGI QHLT SDSD SEVY CDSM EQFG QEES LDSF TSNN GPFQ YYLG GHSS QPME NSGF REDI QVPP GNGN IGNM QVVA VEGK GEVK HGGE DGRN NSGA PHRE KRGG ETDE FSNV RRGR GHRM QHLS EGTK GRQV GSGG DGER WGSD RGSR GSLN EQI |
| Gene ID: | 91452 |
| Uniprot: | Q5T8D3 |
| Cellular Location: | Peroxisome membrane, Single-pass membrane protein |
| Calculated MW: | 46kDa/54kDa/58kDa/60kDa |
| Observed MW: | 70kDa |
| Synonyms: | ACBD5 |
| Background: | This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ACBD5: Binds medium- and long-chain acyl-CoA esters. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid-binding; Membrane protein, integral Chromosomal Location of Human Ortholog: 10p12.1 Cellular Component: membrane; nucleoplasm; nucleus; peroxisomal membrane; peroxisome Biological Process: cellular lipid metabolic process; macroautophagy; peroxisome degradation |
| NCBI Summary: | This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q5T8D3 |
| NCBI GenInfo Identifier: | 74745508 |
| NCBI Gene ID: | 91452 |
| NCBI Accession: | Q5T8D3.1 |
| UniProt Secondary Accession: | Q5T8D3,Q5T8D4, Q5T8E1, Q5T8E2, Q86UV1, Q8N6E3, Q9UFB5 B3KQ56, D3DRW0, |
| UniProt Related Accession: | Q5T8D3 |
| Molecular Weight: | 60kDa |
| NCBI Full Name: | Acyl-CoA-binding domain-containing protein 5 |
| NCBI Synonym Full Names: | acyl-CoA binding domain containing 5 |
| NCBI Official Symbol: | ACBD5 |
| NCBI Protein Information: | acyl-CoA-binding domain-containing protein 5 |
| UniProt Protein Name: | Acyl-CoA-binding domain-containing protein 5 |
| Protein Family: | Acyl-CoA-binding domain-containing protein |
| UniProt Gene Name: | ACBD5 |
 | Western blot analysis of extracts of various cell lines, using ACBD5 antibody (CAB10595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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