Anti-AMACR Antibody (CAB20825)
- SKU:
- CAB20825
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- Alexa Fluor 488 conjugated IgG
Description
| Antibody Name: | Anti-AMACR Antibody (CAB20825) |
| Antibody SKU: | CAB20825 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human AMACR (NP_055139.4). |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LNCaP, Rat liver, Rat kidney, Mouse liver, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human AMACR (NP_055139.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALQ GISV VELS GLAP GPFC AMVL ADFG ARVV RVDR PGSR YDVS RLGR GKRS LVLD LKQP RGAA VLRR LCKR SDVL LEPF RRGV MEKL QLGP EILQ RENP RLIY ARLS GFGQ SGSF CRLA GHDI NYLA LSGV LSKI GR |
| Cellular Location: | Mitochondrion, Peroxisome |
| Calculated MW: | 22kDa/25kDa/42kDa/43kDa |
| Observed MW: | 42KDa |
| Synonyms: | AMACR, AMACRD, CBAS4, P504S, RACE, RM |
| Background: | This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. |
 | Immunohistochemistry of paraffin-embedded human kidney using AMACR Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human prostate cancer using AMACR Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human benign prostatic hyperplasia (negative control sample) using AMACR Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human prostate cancer using AMACR Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human kidney using AMACR Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using AMACR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using AMACR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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