Autophagy Antibodies
Anti-Ambra1 Antibody (CAB1083)
- SKU:
- CAB1083
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-Ambra1 Antibody |
| Antibody SKU: | CAB1083 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HeLa, Jurkat, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GAAH TSSP QPST SRGL LPEA GQLA ERGL SPRT ASWD QPGT PGRE PTQP TLPS SSPV PIPV SLPS AEGP TLHC ELTN NNHL LDGG SSRG DAAG PRGE PRNR |
| Gene ID: | 55626 |
| Uniprot: | Q9C0C7 |
| Cellular Location: | Cytoplasmic vesicle, autophagosome |
| Calculated MW: | 84kDa/132kDa/135kDa/139kDa/142kDa |
| Observed MW: | 143kDa |
| Synonyms: | AMBRA1, DCAF3, WDR94, FLJ20294 |
| Background: |
| UniProt Protein Function: | AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy; Vesicle Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; autophagic vacuole; phagocytic vesicle; axoneme; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: neural tube development; negative regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; autophagy; negative regulation of neuron apoptosis; cell differentiation |
| UniProt Code: | Q9C0C7 |
| NCBI GenInfo Identifier: | 166215833 |
| NCBI Gene ID: | 55626 |
| NCBI Accession: | Q9C0C7.2 |
| UniProt Secondary Accession: | Q9C0C7,A2AH22, |
| UniProt Related Accession: | Q9C0C7 |
| Molecular Weight: | |
| NCBI Full Name: | Activating molecule in BECN1-regulated autophagy protein 1 |
| NCBI Synonym Full Names: | autophagy and beclin 1 regulator 1 |
| NCBI Official Symbol: | AMBRA1 |
| NCBI Official Synonym Symbols: | DCAF3; WDR94 |
| NCBI Protein Information: | activating molecule in BECN1-regulated autophagy protein 1 |
| UniProt Protein Name: | Activating molecule in BECN1-regulated autophagy protein 1 |
| Protein Family: | Activating molecule in BECN1-regulated autophagy protein |
| UniProt Gene Name: | AMBRA1 |
| UniProt Entry Name: | AMRA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Ambra1 Antibody (CAB1083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens). |
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