Epigenetics & Nuclear Signaling Antibodies 1

Anti-ATF3 Antibody (CAB13469)

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SKU:
CAB13469
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ATF3 Antibody
Antibody SKU:CAB13469
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, RAW264.7, RAW264.7+LPS
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMLQ HPGQ VSAS EVSA SAIV PCLS PPGS LVFE DFAN LTPF VKEE LRFA IQNK HLCH RMSS ALES VTVS DRPL GVSI TKAE VAPE EDER KKRR RERN KIAA AKCR NKKK EKTE CLQK ESEK LESV NAEL KAQI EELK NEKQ HLIY MLNL HRPT CIVR AQNG RTPE DERN LFIQ QIKE GTLQ S
Gene ID:467
Uniprot:P18847
Cellular Location:Nucleus
Calculated MW:12kDa/13kDa/14kDa/15kDa/20kDa
Observed MW:21kDa
Synonyms:ATF3
Background:This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
UniProt Protein Function:ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
UniProt Code:P18847
NCBI GenInfo Identifier:1168543
NCBI Gene ID:467
NCBI Accession:P18847.2
UniProt Secondary Accession:P18847,Q5VTZ2, Q6ICQ9, Q7Z566, Q8WYM6,
UniProt Related Accession:P18847
Molecular Weight:14,314 Da
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-3
NCBI Synonym Full Names:activating transcription factor 3
NCBI Official Symbol:ATF3  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-3
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-3
UniProt Synonym Protein Names:Activating transcription factor 3
Protein Family:Activating transcription factor
UniProt Gene Name:ATF3  
UniProt Entry Name:ATF3_HUMAN
Anti-ATF3 Antibody (CAB13469)Western blot analysis of extracts of various cell lines, using ATF3 antibody (CAB13469) at 1:1000 dilution. Raw264.7 cells were treated by LPS (1 ug/mL) at 37'C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-ATF3 Antibody (CAB13469)Immunofluorescence analysis of U-2 OS cells using ATF3 antibody (CAB13469) at dilution of 1:100. Blue: DAPI for nuclear staining.
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