Autophagy Antibodies
Anti-ATG2B Antibody (CAB8498)
- SKU:
- CAB8498
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-ATG2B Antibody |
| Antibody SKU: | CAB8498 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1360-1600 of human ATG2B (NP_060506.5). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, MCF7, A-549, SW480, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1360-1600 of human ATG2B (NP_060506.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IQYI ASYG DLQT PNKA DMKP GAFQ RRSK VDSS GRSS SRGP VLPE ADQQ MLRD LMSD AMEE IDMQ QGTS SVKP QANG VLDE KSQI QEPC CSDL FLFP DESG NVSQ ESGP TYAS FSHH FISD AMTG VPTE NDDF CILF APKA AMQE KEEE PVIK IMVD DAIV IRDN YFSL PVNK TDTS KAPL HFPI PVIR YVVK EVSL VWHL YGGK DFGT VPPT SPAK SYIS PHSS PSHT PTRH GRNT VCGG K |
| Gene ID: | 55102 |
| Uniprot: | Q96BY7 |
| Cellular Location: | Lipid droplet, Peripheral membrane protein, Preautophagosomal structure membrane |
| Calculated MW: | 232kDa |
| Observed MW: | 270kDa |
| Synonyms: | ATG2B, C14orf103 |
| Background: |
| UniProt Protein Function: | ATG2B: Required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Belongs to the ATG2 family. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 14q32.2 Cellular Component: extrinsic to membrane Biological Process: autophagic vacuole formation; mitochondrion degradation |
| NCBI Summary: | This gene encodes a protein required for autophagy. The encoded protein is involved in autophagosome formation. A germline duplication of a region that includes this gene is associated with predisposition to myeloid malignancies. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q96BY7 |
| NCBI GenInfo Identifier: | 308153682 |
| NCBI Gene ID: | 55102 |
| NCBI Accession: | Q96BY7.5 |
| UniProt Secondary Accession: | Q96BY7,Q6ZRE7, Q96DQ3, Q9NW80, |
| UniProt Related Accession: | Q96BY7 |
| Molecular Weight: | 232,763 Da |
| NCBI Full Name: | Autophagy-related protein 2 homolog B |
| NCBI Synonym Full Names: | autophagy related 2B |
| NCBI Official Symbol: | ATG2B |
| NCBI Official Synonym Symbols: | C14orf103 |
| NCBI Protein Information: | autophagy-related protein 2 homolog B |
| UniProt Protein Name: | Autophagy-related protein 2 homolog B |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG2B |
| UniProt Entry Name: | ATG2B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG2B antibody (CAB8498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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