Cell Biology Antibodies 9

Anti-CD63 Antibody (CAB5271)

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SKU:
CAB5271
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CD63 Antibody
Antibody SKU:CAB5271
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, RAW264.7, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AGYV FRDK VMSE FNNN FRQQ MENY PKNN HTAS ILDR MQAD FKCC GAAN YTDW EKIP SMSK NRVP DSCC INVT VGCG INFN EKAI HKEG CVEK IGGW LRKN V
Gene ID:967
Uniprot:P08962
Cellular Location:Cell membrane, Endosome, Late endosome membrane, Lysosome membrane, Melanosome, Multi-pass membrane protein, multivesicular body
Calculated MW:17kDa/23kDa/25kDa
Observed MW:30-60kDa
Synonyms:CD63, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 antigen, CD63
Background:The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.
UniProt Protein Function:Function: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
UniProt Protein Details:

Subcellular location: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note: Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Ref.3 Ref.13 Ref.15 Ref.17 Ref.18

Tissue specificity: Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Miscellaneous: Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.

Sequence similarities: Belongs to the tetraspanin (TM4SF) family.

NCBI Summary:The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
UniProt Code:P08962
NCBI GenInfo Identifier:383872547
NCBI Gene ID:967
NCBI Accession:NP_001244330.1
UniProt Secondary Accession:P08962,Q5TZP3, Q8N6Z9, Q9UCG6, F8VZE2,
UniProt Related Accession:P08962
Molecular Weight:
NCBI Full Name:CD63 antigen isoform D
NCBI Synonym Full Names:CD63 molecule
NCBI Official Symbol:CD63  
NCBI Official Synonym Symbols:MLA1; ME491; LAMP-3; OMA81H; TSPAN30  
NCBI Protein Information:CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3
UniProt Protein Name:CD63 antigen
UniProt Synonym Protein Names:Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30
Protein Family:CD63 antigen
UniProt Gene Name:CD63  
UniProt Entry Name:CD63_HUMAN
Anti-CD63 Antibody (CAB5271)Western blot analysis of extracts of various cell lines, using CD63 antibody (CAB5271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-CD63 Antibody (CAB5271)Immunohistochemistry of paraffin-embedded rat brain using CD63 Rabbit pAb (CAB5271) at dilution of 1:100 (40x lens).
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