Epigenetics & Nuclear Signaling Antibodies 3
Anti-CENPB Antibody (CAB16817)
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- SKU:
- CAB16817
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CENPB Antibody |
| Antibody SKU: | CAB16817 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human CENPB (NP_001801.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human CENPB (NP_001801.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TLHF LEGG EDSD SDSE EEDD EEED DEDE DDDD DEED GDEV PVPS FGEA MAYF AMVK RYLT SFPI DDRV QSHI LHLE HDLV HVTR KNHA RQAG VRGL GHQS |
| Gene ID: | 1059 |
| Uniprot: | P07199 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to figures |
| Synonyms: | CENPB |
| Background: | This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. |
| UniProt Protein Function: | CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 20p13 Cellular Component: chromosome; nucleus; chromosome, pericentric region Molecular Function:centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding Biological Process: regulation of transcription, DNA-dependent |
| UniProt Code: | P07199 |
| NCBI GenInfo Identifier: | 29863 |
| NCBI Gene ID: | |
| NCBI Accession: | CAA28918.1 |
| Molecular Weight: | 80kD |
| NCBI Full Name: | CENP-B |
| UniProt Protein Name: | Major centromere autoantigen B |
| UniProt Synonym Protein Names: | Centromere protein B |
| Protein Family: | CENP-B homolog protein |
| UniProt Gene Name: | CENPB |
| UniProt Entry Name: | CENPB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CENPB antibody (CAB16817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
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