Developmental Biology
Anti-DAZ1 Antibody (CAB5310)
- SKU:
- CAB5310
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-DAZ1 Antibody |
| Antibody SKU: | CAB5310 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-530 of human DAZ1 (NP_004072.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 22Rv1, ES-2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-530 of human DAZ1 (NP_004072.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NSTI SREA STQS SSAA ASQG WVLP EGKI VPNT VFVG GIDA RMDE TEIG SCFG RYGS VKEV KIIT NRTG VSKG YGFV SFVN DVDV QKIV GSQI HFHG KKLK LGPA IRKQ KLCA RHVQ PRPL VVNP PPPP QFQN VWRN PNTE TYLQ PQIT PNPV TQHV QSAA NPET PNST ISRE ASTQ SSSA AASQ GWVL PEG |
| Gene ID: | 1617 |
| Uniprot: | Q9NQZ3 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 67kDa/82kDa |
| Observed MW: | 83kDa |
| Synonyms: | DAZ1, DAZ, SPGY |
| Background: | This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains. |
| UniProt Protein Function: | DAZ1: RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells. Defects in DAZ1 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2). It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility. AZFc deletions in the Yq11.23 region including the DAZ genes are the most common known genetic cause of human male infertility. Belongs to the RRM DAZ family. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: Yq11.223 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; translation activator activity; RNA binding; nucleotide binding Biological Process: positive regulation of translational initiation; multicellular organismal development; spermatogenesis; cell differentiation Disease: Spermatogenic Failure, Y-linked, 2 |
| NCBI Summary: | This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQZ3 |
| NCBI GenInfo Identifier: | 44887841 |
| NCBI Gene ID: | 1617 |
| NCBI Accession: | Q9NQZ3.2 |
| UniProt Secondary Accession: | Q9NQZ3,Q1RMF9, Q9NQZ4, |
| UniProt Related Accession: | Q9NQZ3 |
| Molecular Weight: | 744 |
| NCBI Full Name: | Deleted in azoospermia protein 1 |
| NCBI Synonym Full Names: | deleted in azoospermia 1 |
| NCBI Official Symbol: | DAZ1 |
| NCBI Official Synonym Symbols: | DAZ; SPGY |
| NCBI Protein Information: | deleted in azoospermia protein 1 |
| UniProt Protein Name: | Deleted in azoospermia protein 1 |
| Protein Family: | Deleted in azoospermia protein |
| UniProt Gene Name: | DAZ1 |
| UniProt Entry Name: | DAZ1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAZ1 antibody (CAB5310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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