Developmental Biology
Anti-DIAPH2 Antibody (CAB10209)
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- SKU:
- CAB10209
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-DIAPH2 Antibody |
| Antibody SKU: | CAB10209 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DIAPH2 (NP_009293.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, SW620, NCI-H460, LO2, 293T, Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DIAPH2 (NP_009293.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEQP GAAA SGAG GGSE EPGG GRSN KRSA GNRA ANEE ETKN KPKL NIQI KTLA DDVR DRIT SFRK STVK KEKP LIQH PIDS QVAM SEFP AAQP LYDE RSLN LSEK EVLD LFEK MMED MNLN |
| Gene ID: | 1730 |
| Uniprot: | O60879 |
| Cellular Location: | Cytoplasm, Early endosome, cytosol |
| Calculated MW: | 124kDa/125kDa |
| Observed MW: | 125kDa |
| Synonyms: | DIAPH2, DIA, DIA2, DRF2, POF, POF2, POF2A |
| Background: | The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | Diaphanous-2: Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. Isoform 3 interacts with RHOD in the GTP-bound form. Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. Belongs to the formin homology family. Diaphanous subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xq21.33 Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleolus Molecular Function:receptor binding Biological Process: cytokinesis; female gamete generation Disease: Premature Ovarian Failure 2a |
| NCBI Summary: | The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60879 |
| NCBI GenInfo Identifier: | 6166119 |
| NCBI Gene ID: | 1730 |
| NCBI Accession: | O60879.1 |
| UniProt Secondary Accession: | O60879,O60878, Q8WX06, Q8WX48, Q9UJL2, A6NG19, |
| UniProt Related Accession: | O60879 |
| Molecular Weight: | 125,043 Da |
| NCBI Full Name: | Protein diaphanous homolog 2 |
| NCBI Synonym Full Names: | diaphanous related formin 2 |
| NCBI Official Symbol: | DIAPH2 |
| NCBI Official Synonym Symbols: | DIA; POF; DIA2; DRF2; POF2 |
| NCBI Protein Information: | protein diaphanous homolog 2 |
| UniProt Protein Name: | Protein diaphanous homolog 2 |
| UniProt Synonym Protein Names: | Diaphanous-related formin-2; DRF2 |
| Protein Family: | Protein diaphanous |
| UniProt Gene Name: | DIAPH2 |
| UniProt Entry Name: | DIAP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DIAPH2 antibody (CAB10209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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