KO Validated Antibodies 2

Anti-EIF4G2 Antibody (CAB19990)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19990
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-EIF4G2 Antibody [KO Validated]
Antibody SKU:CAB19990
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human EIF4G2.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:A synthetic peptide of human EIF4G2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1982
Uniprot:P78344
Cellular Location:
Calculated MW:98kDa/102kDa
Observed MW:97KDa
Anti-EIF4G2 Antibody [KO Validated] (CAB19990)Western blot analysis of extracts from normal (control) and EIF4G2 knockout (KO) 293T cells, using EIF4G2 antibody (CAB19990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Synonyms:EIF4G2, AAG1, DAP5, NAT1, P97
Background:Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
UniProt Protein Function:EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cell death; cytokine and chemokine mediated signaling pathway; translational initiation; cell cycle arrest; regulation of translational initiation

NCBI Summary:Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P78344
NCBI GenInfo Identifier:30315906
NCBI Gene ID:1982
NCBI Accession:P78344.1
UniProt Secondary Accession:P78344,O60877, P78404, Q0VH00, Q0VH01, Q2NKW9, Q49A79 Q53EU1, Q58EZ2, Q8NI71, Q96C16,
UniProt Related Accession:P78344
Molecular Weight:907
NCBI Full Name:Eukaryotic translation initiation factor 4 gamma 2
NCBI Synonym Full Names:eukaryotic translation initiation factor 4 gamma, 2
NCBI Official Symbol:EIF4G2  
NCBI Official Synonym Symbols:P97; AAG1; DAP5; NAT1  
NCBI Protein Information:eukaryotic translation initiation factor 4 gamma 2; DAP-5; EIF4G2; eIF4G 2; eIF-4G 2; eIF-4-gamma 2; aging-associated protein 1; death-associated protein 5; eukaryotic translation initiation factor 4G-like 1
UniProt Protein Name:Eukaryotic translation initiation factor 4 gamma 2
UniProt Synonym Protein Names:Death-associated protein 5; DAP-5; p97
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF4G2  
UniProt Entry Name:IF4G2_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products