Developmental Biology
Anti-ELSPBP1 Antibody (CAB2151)
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- SKU:
- CAB2151
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-ELSPBP1 Antibody |
| Antibody SKU: | CAB2151 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human ELSPBP1 (NP_071425.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HepG2, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human ELSPBP1 (NP_071425.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTRW SSYL LGWT TFLL YSYE SSGG MHEE CVFP FTYK GSVY FTCT HIHS LSPW CATR AVYN GQWK YCQS EDYP RCIF PFIY RGKA YNSC ISQG SFLG SLWC SVTS VFDE KQQW KFCE TNEY GGNS LRKP CIFP SIYR NNVV SDCM EDES NKLW CPTT ENMD KDGK WSFC ADTR ISAL VPGF PCHF PFNY KNKN YFNC TNEG SKEN LVWC ATSY NYDQ DHTW VYC |
| Gene ID: | 64100 |
| Uniprot: | Q96BH3 |
| Cellular Location: | Secreted |
| Calculated MW: | 26kDa |
| Observed MW: | 34kDa |
| Synonyms: | ELSPBP1, E12, EDDM12, EL149, HE12 |
| Background: | The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. |
| UniProt Protein Function: | ELSPBP1: Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity. Belongs to the seminal plasma protein family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19q13.33 Cellular Component: extracellular region Biological Process: single fertilization |
| NCBI Summary: | The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96BH3 |
| NCBI GenInfo Identifier: | 296434494 |
| NCBI Gene ID: | 64100 |
| NCBI Accession: | Q96BH3.2 |
| UniProt Related Accession: | Q96BH3 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | Epididymal sperm-binding protein 1 |
| NCBI Synonym Full Names: | epididymal sperm binding protein 1 |
| NCBI Official Symbol: | ELSPBP1 |
| NCBI Official Synonym Symbols: | E12; HE12; EL149; EDDM12 |
| NCBI Protein Information: | epididymal sperm-binding protein 1 |
| UniProt Protein Name: | Epididymal sperm-binding protein 1 |
| UniProt Synonym Protein Names: | Epididymal secretory protein 12; hE12 |
| Protein Family: | Epididymal sperm-binding protein |
| UniProt Gene Name: | ELSPBP1 |
| UniProt Entry Name: | ESPB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody (CAB2151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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