Name: Anti-FOXC1 Antibody (CAB2924)
Brand: Cardiovascular Antibodies
SKU: CAB2924
Availability: OutOfStock

Antibody Name:	Anti-FOXC1 Antibody
Antibody SKU:	CAB2924
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 404-553 of human FOXC1 (NP_001444.2).

Application:	WB IHC
Recommended Dilution:	WB 1:100 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, SKOV3, Mouse kidney, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 404-553 of human FOXC1 (NP_001444.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAGE RGGH LQGA PGGA GGSA VDDP LPDY SLPP VTSS SSSS LSHG GGGG GGGG GQEA GHHP AAHQ GRLT SWYL NQAG GDLG HLAS AAAA AAAA GYPG QQQN FHSV REMF ESQR IGLN NSPV NGNS SCQM AFPS SQSL YRTS GAFV YDCS KF
Gene ID:	2296
Uniprot:	Q12948
Cellular Location:	Nucleus
Calculated MW:	56kDa
Observed MW:	70kDa

Synonyms:

FOXC1, ARA, ASGD3, FKHL7, FREAC-3, FREAC3, IGDA, IHG1, IRID1, RIEG3

Background:

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.

UniProt Protein Function:	FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding; Cell cycle regulation; Cell development/differentiation Chromosomal Location of Human Ortholog: 6p25 Cellular Component: cytoplasm; nuclear heterochromatin; nucleoplasm; nucleus Molecular Function:DNA bending activity; DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: cell migration; cell proliferation; eye development; heart development; mesenchymal cell development; negative regulation of angiogenesis; negative regulation of mitotic cell cycle; odontogenesis of dentine-containing teeth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1
NCBI Summary:	This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]
UniProt Code:	Q12948
NCBI GenInfo Identifier:	13638267
NCBI Gene ID:	2296
NCBI Accession:	Q12948.3
UniProt Secondary Accession:	Q12948,Q86UP7, Q9BYM1, Q9NUE5, Q9UDD0, Q9UP06,
UniProt Related Accession:	Q12948
Molecular Weight:	56,789 Da
NCBI Full Name:	Forkhead box protein C1
NCBI Synonym Full Names:	forkhead box C1
NCBI Official Symbol:	FOXC1
NCBI Official Synonym Symbols:	ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
NCBI Protein Information:	forkhead box protein C1
UniProt Protein Name:	Forkhead box protein C1
UniProt Synonym Protein Names:	Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FREAC-3
Protein Family:	Forkhead box protein
UniProt Gene Name:	FOXC1
UniProt Entry Name:	FOXC1_HUMAN

Western blot analysis of extracts of various cell lines, using FOXC1 antibody (CAB2924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

ELISA Kits

Human FoxC1 / Forkhead Box Protein C1 ELISA Kit

Human FOXC1 (Forkhead Box Protein C1) CLIA Kit (HUES00646)

Human FOXC1 (Forkhead Box Protein C1) ELISA Kit (HUES02192)

FOXC1/2 Transcription Factor Activity Assay

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)