Cardiovascular Antibodies
Anti-IREB2 Antibody (CAB6382)
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- SKU:
- CAB6382
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-IREB2 Antibody |
| Antibody SKU: | CAB6382 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IREB2 (NP_004127.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IREB2 (NP_004127.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDAP KAGY AFEY LIET LNDS SHKK FFDV SKLG TKYD VLPY SIRV LLEA AVRN CDGF LMKK EDVM NILD WKTK QSNV EVPF FPAR VLLQ DFTG IPAM VDFA AMRE AVKT LGGD PEKV HPAC PTDL TVDH SLQI DFSK CAIQ NAPN PGGG DLQK AGKL SPLK VQPK KLPC RGQT TCRG SCDS GELG RNSG TFSS QIEN TPIL CPFH LQPV PEPE TVLK NQEV EFGR NRER LQFF KWSS RVFK NVAV IPPG TG |
| Gene ID: | 3658 |
| Uniprot: | P48200 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 37kDa/105kDa |
| Observed MW: | 105kDa |
| Synonyms: | IREB2, ACO3, IRP2, IRP2AD |
| Background: |
| UniProt Protein Function: | IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 15q25.1 Cellular Component: mitochondrion; cytoplasm; cytosol Molecular Function:protein binding; iron-responsive element binding; translation repressor activity; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; osteoclast differentiation; response to iron(II) ion; iron ion transport; protoporphyrinogen IX biosynthetic process; aging; intestinal absorption; post-embryonic development |
| UniProt Code: | P48200 |
| NCBI GenInfo Identifier: | 308153591 |
| NCBI Gene ID: | 3658 |
| NCBI Accession: | P48200.3 |
| UniProt Secondary Accession: | P48200,Q13095, Q1HE21, Q59FQ7, Q8WVK6, Q9UF17, A8KAC7 E1CJT9, H0YKU0, |
| UniProt Related Accession: | P48200 |
| Molecular Weight: | 963 |
| NCBI Full Name: | Iron-responsive element-binding protein 2 |
| NCBI Synonym Full Names: | iron-responsive element binding protein 2 |
| NCBI Official Symbol: | IREB2 |
| NCBI Official Synonym Symbols: | ACO3; IRP2; IRP2AD |
| NCBI Protein Information: | iron-responsive element-binding protein 2; IRE-BP 2; iron regulatory protein 2 |
| UniProt Protein Name: | Iron-responsive element-binding protein 2 |
| UniProt Synonym Protein Names: | Iron regulatory protein 2; IRP2 |
| Protein Family: | Iron-responsive element-binding protein |
| UniProt Gene Name: | IREB2 |
| UniProt Entry Name: | IREB2_HUMAN |
 | Western blot analysis of extracts of 293T cells, using IREB2 antibody (CAB6382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Western blot analysis of extracts of various cell lines, using IREB2 antibody (CAB6382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of A549 cells using IREB2 antibody (CAB6382). Blue: DAPI for nuclear staining. |
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