Autophagy Antibodies
Anti-LC3A / LC3B Antibody (CAB15591)
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- SKU:
- CAB15591
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-LC3A / LC3B Antibody |
| Antibody SKU: | CAB15591 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE SEKD EDGF LYMV YASQ ETFG MKLS V |
| Gene ID: | 84557/81631 |
| Uniprot: | Q9GZQ8/Q9H492 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton |
| Calculated MW: | 14kDa |
| Observed MW: | 14kDa, 16kDa |
| Synonyms: | MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3A/LC3B |
| Background: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| NCBI Summary: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9GZQ8 |
| NCBI GenInfo Identifier: | 17433141 |
| NCBI Gene ID: | 81631 |
| NCBI Accession: | Q9GZQ8.3 |
| UniProt Related Accession: | Q9GZQ8 |
| Molecular Weight: | |
| NCBI Full Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| NCBI Synonym Full Names: | microtubule associated protein 1 light chain 3 beta |
| NCBI Official Symbol: | MAP1LC3B |
| NCBI Official Synonym Symbols: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| NCBI Protein Information: | microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Protein Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Synonym Protein Names: | Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta |
| UniProt Gene Name: | MAP1LC3B |
| UniProt Entry Name: | MLP3B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (CAB15591) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 uM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human mammary cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using LC3A / LC3B antibody (CAB15591) at dilution of 1:100. Hela cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining. |
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