Autophagy Antibodies
Anti-MAP1LC3C Antibody (CAB8295)
(No reviews yet)
Write a Review
- SKU:
- CAB8295
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Description
| Antibody Name: | MAP1LC3C Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8295 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human MAP1LC3C (NP_001004343.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, OVCAR3, HepG2, 293T, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human MAP1LC3C (NP_001004343.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPPP QKIP SVRP FKQR KSLA IRQE EVAG IRAK FPNK IPVV VERY PRET FLPP LDKT KFLV PQEL TMTQ FLSI IRSR MVLR ATEA FYLL VNNK SLVS MSAT MAEI YRDY KDED GFVY MTYA SQET FGCL ESAA PRDG SSLE DRPC NPL |
| Gene ID: | 440738 |
| Uniprot: | Q9BXW4 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton |
| Calculated MW: | 16kDa |
| Observed MW: | 17kDa |
| Synonyms: | MAP1LC3C, ATG8J, LC3C |
| Background: |
| UniProt Protein Function: | LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. |
| UniProt Protein Details: | Protein type:Vesicle; Autophagy; Microtubule-binding; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 1q43 Cellular Component: microtubule; extrinsic to membrane; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol Molecular Function:protein binding; microtubule binding; GABA receptor binding Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation |
| NCBI Summary: | Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q9BXW4 |
| NCBI GenInfo Identifier: | 84028113 |
| NCBI Gene ID: | 440738 |
| NCBI Accession: | Q9BXW4.1 |
| UniProt Secondary Accession: | Q9BXW4,A0PJY8, A2RUP0, |
| UniProt Related Accession: | Q9BXW4 |
| Molecular Weight: | 16,852 Da |
| NCBI Full Name: | Microtubule-associated proteins 1A/1B light chain 3C |
| NCBI Synonym Full Names: | microtubule-associated protein 1 light chain 3 gamma |
| NCBI Official Symbol: | MAP1LC3C |
| NCBI Official Synonym Symbols: | LC3C; ATG8J |
| NCBI Protein Information: | microtubule-associated proteins 1A/1B light chain 3C; LC3-like protein 2; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; autophagy-related protein LC3 C; autophagy-related ubiquitin-like modifier LC3 C |
| UniProt Protein Name: | Microtubule-associated proteins 1A/1B light chain 3C |
| UniProt Synonym Protein Names: | Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma |
| Protein Family: | Microtubule-associated protein |
| UniProt Gene Name: | MAP1LC3C |
| UniProt Entry Name: | MLP3C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAP1LC3C antibody (CAB8295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
View AllClose
Related Products
Anti-RANBP3 Antibody (CAB0092)
OverviewProduct Name:RANBP3 Rabbit mAbProduct Code:CAB0092Size:20uL, 50uL, 100uLSynonyms:RANBP3, ran-binding protein 3
Anti-QK1 Antibody (CAB0193)
OverviewProduct Name:QK1 Rabbit mAbProduct Code:CAB0193Size:20uL, 50uL, 100uLSynonyms:QKI, Hqk, QK, QK1, QK3, hqkI, protein quaking
Anti-DEK Antibody (CAB0315)
OverviewProduct Name:DEK Rabbit mAbProduct Code:CAB0315Size:20uL, 50uL, 100uLSynonyms:D6S231EApplications:WB,
Anti-EXOSC7 Antibody (CAB0501)
OverviewProduct Name:EXOSC7 Rabbit mAbProduct Code:CAB0501Size:20uL, 50uL, 100uLSynonyms:EXOSC7, EAP1, RRP42, Rrp42p, hRrp42p, p8, exoso
Anti-Olig3 Antibody (CAB0600)
OverviewProduct Name:Olig3 Rabbit mAbProduct Code:CAB0600Size:20uL, 50uL, 100uLSynonyms:OLIG3, Bhlhb7, bHLHe20, oligodendrocyte transcri