Anti-MonoMethyl-Histone H3-K79 Antibody (CAB20821)
- SKU:
- CAB20821
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Donkey
- Isotype:
- Rhodamine (TRITC) conjugated IgG
Description
| Antibody Name: | Anti-MonoMethyl-Histone H3-K79 Antibody (CAB20821) |
| Antibody SKU: | CAB20821 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic monomethylated peptide around K79 of human histone H3 (NP_003520.1). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 ChIP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, C6, H3 |
| Immunogen: | A synthetic monomethylated peptide around K79 of human histone H3 (NP_003520.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QDFK T |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 17KDa |
| Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K79 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
 | Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K79 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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