Anti-Phospho-MELK-T167/S171 Antibody (CABP1175)

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SKU:
CABP1175
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MELK
Synonyms:
HPK38
Synonyms:
maternal embryonic leucine zipper kinase
Research Area:
Cell Death

Description

Product Name:Phospho-MELK-T167/S171 Rabbit pAb
Product Code:CABP1175
Size:20uL, 50uL, 100uL
Synonyms:MELK, HPK38, maternal embryonic leucine zipper kinase
Applications:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A phospho synthetic peptide corresponding to residues surrounding T167/S171 of Human MELK.
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human
Immunogen:A phospho synthetic peptide corresponding to residues surrounding T167/S171 of Human MELK.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9833
Uniprot:Q14680
Cellular Location:Cell membrane, Peripheral membrane protein
Calculated MW:52kDa/59kDa/66kDa/69kDa/70kDa/71kDa/74kDa
Observed MW:Refer to figures
UniProt Protein Function:MELK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; EC 2.7.10.2; RNA splicing; EC 2.7.11.1; CAMK group; CAMKL family; MELK subfamily

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: cell cortex; membrane; nucleus; plasma membrane

Molecular Function:calcium ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: apoptosis; cell proliferation; G2/M transition of mitotic cell cycle; hemopoiesis; positive regulation of apoptosis; protein amino acid autophosphorylation

UniProt Code:Q14680
NCBI GenInfo Identifier:50400857
NCBI Gene ID:9833
NCBI Accession:Q14680.3
UniProt Secondary Accession:Q14680,A6P3A7, A6P3A8, B1AMQ6, B7Z1E6, B7Z5M5, B7Z6Q7 B7Z6R8, B7Z6Y0, B7Z7Q1, D3DRP8, F5H0Y0,
UniProt Related Accession:Q14680
Molecular Weight:69,116 Da
NCBI Full Name:Maternal embryonic leucine zipper kinase
NCBI Synonym Full Names:maternal embryonic leucine zipper kinase
NCBI Official Symbol:MELK  
NCBI Official Synonym Symbols:HPK38  
NCBI Protein Information:maternal embryonic leucine zipper kinase
UniProt Protein Name:Maternal embryonic leucine zipper kinase
UniProt Synonym Protein Names:Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK (EC:2.7.10.2)
Protein Family:Maternal embryonic leucine zipper kinase
UniProt Gene Name:MELK  
UniProt Entry Name:MELK_HUMAN
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