Protein Tags
Anti-Rabbit anti GST-Tag Polyclonal Antibody (CABE006)
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- SKU:
- CABE006
- Product Type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Description
| Antibody Name: | Anti-Rabbit anti GST-Tag Polyclonal Antibody |
| Antibody SKU: | CABE006 |
| Antibody Size: | 100 uL |
| Application: | WB IF IP |
| Reactivity: | |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein. |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:20 - 1:50 IP 1:20 - 1:50 |
| Reactivity: | |
| Positive Samples: | GST fusion protein |
| Immunogen: | Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSPI LGYW KIKG LVQP TRLL LEYL EEKY EEHL YERD EGDK WRNK KFEL GLEF PNLP YYID GDVK LTQS MAII RYIA DKHN MLGG CPKE RAEI SMLE GAVL DIRY GVSR IAYS KDFE TLKV DFLS KLPE MLKM FEDR LCHK TYLN GDHV THPD FMLY DALD VVLY MDPM CLDA FPKL VCFK KRIE AIPQ IDKY LKSS KYIA WPLQ GWQA TFGG GDHP PK |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | 26kDa |
| Observed MW: | Refer to Figures |
 | Western blot analysis of over-expressed GST protein using GST-tag antibody (CABE006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 50ug extracts of GST protein cells using 3ug Rabbit anti GST-Tag pAb antibody (CABE006). Western blot was performed from the immunoprecipitate using Rabbit anti GST-Tag pAb antibody (CABE006) at a dilition of 1:20000. |
| Synonyms: | GST, GST tag, GST-tag |
| Background: | Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomalalso known as MAPEGproteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. |
