Canine ELISA Kits

Canine MCP-1 / CCL2 ELISA Kit

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SKU:
CNFI00016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P52203
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
MCP-1, CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Reactivity:
Canine

Description

Canine MCP-1 / CCL2 ELISA Kit

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Canine MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

Product Code:

CNFI00016

Size:

96 Assays

Target:

Canine MCP-1

Alias:

MCP-1, CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF

Reactivity:

Canine

Detection Method:

Sandwich ELISA, Double Antibody

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Canine MCP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of Canine MCP-1 in samples.

Matrix

Recovery Range (%)

Average (%)

serum(n=5)

87-99

92

EDTA plasma(n=5)

90-103

97

UFH plasma(n=5)

85-102

93

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Canine MCP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-105%

89-105%

91-105%

EDTA plasma(n=5)

84-101%

86-100%

86-99%

UFH plasma(n=5)

86-100%

84-95%

90-99%

Intra-Assay:

CV <8%

Inter-Assay:

CV <10%

Kit Components

Uniprot:

UniProt Protein Function:

Chemotactic factor that attracts monocytes, but not neutrophils. Important factor in the course of the inflammatory reaction to reperfusion of the previously ischemic myocardium. May play a significant role in monocyte trafficking into the reperfused myocardium.

UniProt Code:

NCBI GenInfo Identifier:

NCBI Gene ID:

NCBI Accession:

UniProt Related Accession:

Molecular Weight:

11,121 Da

NCBI Full Name:

C-C motif chemokine 2

NCBI Official Symbol:

CCL2

Protocol

*Note: Protocols are specific to each batch/lot. For the exact instructions please follow the protocol included in your kit.

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Step Procedure

1.

Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!

2.