Enzymes Recombinant Proteins

Human ADAT2 Recombinant Protein (RPPB1351)

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SKU:
RPPB1351
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q7Z6V5
Research Area:
Enzymes

Description

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Product Name:Human ADAT2 Recombinant Protein
Product Code:RPPB1351
Size:10µg
Species:Human
Target:ADAT2
Synonyms:DEADC1, TAD2.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The ADAT2 (0.5mg/ml) protein contains 20mM Tris-HCl buffer pH-8, 2mM DTT, 50mM NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by Analysis by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR�HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA�EEAVEMLKTF YKQENPNAPK SKVRKKECQK S

Adenosine Deaminase (ADA), also recognized as ADAT2 is an enzyme that takes part in purine metabolism. ADAT2 is necessary for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADAT2 is involved in the deamination of adenosine-34 to inosine in numerous tRNAs. ADAT2 is part of the cytidine and deoxycytidylate deaminase protein family, ADAT2 employs zinc as a cofactor. ADAT2 is a 191 amino acid enzyme that exists as two isoforms formed by alternative splicing events.

Recombinant Human ADAT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (1-191 a.a) and having a molecular mass of 23.2 kDa. ADAT2 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:ADAT2: Probably participates in deamination of adenosine-34 to inosine in many tRNAs. Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.5.4.-Chromosomal Location of Human Ortholog: 6q24.2Molecular Function: tRNA-specific adenosine deaminase activity; zinc ion bindingBiological Process: tRNA wobble adenosine to inosine editing
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q7Z6V5
NCBI GenInfo Identifier:555943775
NCBI Gene ID:134637
NCBI Accession:NP_001273188.1
UniProt Secondary Accession:Q7Z6V5,Q7Z327, Q8IY39, A6NL12, B3KWY3
UniProt Related Accession:Q7Z6V5
Molecular Weight:16,100 Da
NCBI Full Name:tRNA-specific adenosine deaminase 2 isoform b
NCBI Synonym Full Names:adenosine deaminase, tRNA-specific 2
NCBI Official Symbol:ADAT2��
NCBI Official Synonym Symbols:TAD2; DEADC1; dJ20N2; dJ20N2.1��
NCBI Protein Information:tRNA-specific adenosine deaminase 2; adenosine deaminase, tRNA-specific 2, TAD2 homolog; deaminase domain containing 1; deaminase domain-containing protein 1; tRNA-specific adenosine deaminase 2 homolog; tRNA-specific adenosine-34 deaminase subunit ADAT2
UniProt Protein Name:tRNA-specific adenosine deaminase 2
UniProt Synonym Protein Names:Deaminase domain-containing protein 1; tRNA-specific adenosine-34 deaminase subunit ADAT2
Protein Family:tRNA-specific adenosine deaminase
UniProt Gene Name:ADAT2��
UniProt Entry Name:ADAT2_HUMAN
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