Enzymes Recombinant Proteins

Human ADPRH Recombinant Protein (RPPB1359)

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SKU:
RPPB1359
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P54922
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ADPRH Recombinant Protein
Product Code:RPPB1359
Size:20µg
Species:Human
Target:ADPRH
Synonyms:[Protein ADP-ribosylarginine] hydrolase, ADP-ribosylarginine hydrolase, ADP-ribose-L-arginine cleaving enzyme, ADPRH, ARH1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ADPRH solution (0.5mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNRLEETARALYS LGSKEDTVIS L

ADP-ribosylarginine hydrolase (ADPRH) is a member of the ADP-ribosylglycohydrolase family. ADPRH catalyzes the removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. The human ADPRH enzyme is DTT-independent as opposed to the rat and mouse enzymes, which require DTT for maximal activity.

ADPRH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 381 amino acids (1-357) and having a molecular mass of 42.1kDa.ADPRH is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ADPRH: Catalyzes the reverse reaction of mono-ADP-ribosylation. Belongs to the ADP-ribosylglycohydrolase family.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.2.2.19

Chromosomal Location of Human Ortholog: 3q13.31-q13.33

Molecular Function:ADP-ribosylarginine hydrolase activity; magnesium ion binding

Biological Process: protein amino acid de-ADP-ribosylation; protein modification process

NCBI Summary:The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]
UniProt Code:P54922
NCBI GenInfo Identifier:1703392
NCBI Gene ID:141
NCBI Accession:P54922.1
UniProt Secondary Accession:P54922,B2R8H1, D3DN83,
UniProt Related Accession:P54922
Molecular Weight:357
NCBI Full Name:[Protein ADP-ribosylarginine] hydrolase
NCBI Synonym Full Names:ADP-ribosylarginine hydrolase
NCBI Official Symbol:ADPRH��
NCBI Official Synonym Symbols:ARH1��
NCBI Protein Information:ADP-ribosylarginine hydrolase; ADP-ribose-L-arginine cleaving enzyme
UniProt Protein Name:[Protein ADP-ribosylarginine] hydrolase
UniProt Synonym Protein Names:ADP-ribose-L-arginine cleaving enzyme
Protein Family:[Protein ADP-ribosylarginine] hydrolase
UniProt Gene Name:ADPRH��
UniProt Entry Name:ADPRH_HUMAN
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system_update_altDatasheetHuman ADPRH/ARH1 Recombinant Protein ADP-Ribosylarginine Hydrolase (ADPRH) belongs to the ADP-Ribosylglycohydrolase family. ADPRH catalyzes removal of mono-ADP-ribose fr