Name: Human Atherosclerosis Array (20 targets) (SARB0024)
SKU: SARB0024
Availability: OutOfStock

Product Sku:	SARB0024
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	CCL11, CCL2, CCL20, CCL5, CSF1, CSF2, CSF3, GDNF, ICAM1, IFNG\|IL1A, IL1B, IL1R1, IL1R2, LTA, PDGFB, TGFB1, TGFB2, TGFB3, TNF
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362
IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778	IL-1 R2 Gene Symbol: IL1R2 Gene ID: 7850 Accession #: P27930
MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600
TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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