Human Cytokine Array (51 targets) (SARB0044)
- SKU:
- SARB0044
- Product Type:
- Protein Array
- Analytes:
- Adipsin (Complement Factor D)
- Analytes:
- BCAM
- Analytes:
- CD30 (TNFRSF8)
- Analytes:
- CD40 (TNFRSF5)
- Analytes:
- Fc gamma RIIB/C (CD32B/C)
- Analytes:
- Ferritin
- Analytes:
- FLRG
- Analytes:
- Follistatin
- Analytes:
- Furin
- Analytes:
- Galectin-7
- Analytes:
- GDF-15
- Analytes:
- Growth Hormone
- Analytes:
- IL-10 R alpha
- Analytes:
- IL-22
- Analytes:
- IL-28A (IFN-lambda 2)
- Analytes:
- IL-29 (IFN-lambda 1)
- Analytes:
- IL-31
- Analytes:
- Insulin
- Analytes:
- Luteinizing hormone
- Analytes:
- LIMPII
- Analytes:
- LYVE-1
- Analytes:
- Marapsin
- Analytes:
- MICA
- Analytes:
- MICB
- Analytes:
- MMP-2
- Analytes:
- MMP-7
- Analytes:
- MMP-8
- Analytes:
- MMP-10
- Analytes:
- NCAM-1 (CD56)
- Analytes:
- Nidogen-1
- Analytes:
- NrCAM
- Analytes:
- NRG1-beta 1 (HRG1-beta 1)
- Analytes:
- Osteopontin (SPP1)
- Analytes:
- PAI-1
- Analytes:
- Platelet factor 4 (CXCL4)
- Analytes:
- PSA-total
- Analytes:
- RAGE
- Analytes:
- RANK (TNFRSF11A)
- Analytes:
- Resistin
- Analytes:
- SAA (Serum Amyloid A)
- Analytes:
- Siglec-9
- Analytes:
- TACE
- Analytes:
- TIM-1 (KIM-1)
- Analytes:
- TRAIL R2 (TNFRSF10B/DR5)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0044 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 51 |
| Gene Symbols: | ADAM17, AGER, BCAM, CD40, CFD, CGA, EDA2R, FCGR2B, FST, FSTL3|FTH1, FURIN, GDF15, GH1, HAVCR1, IFNL1, IFNL2, IL10RA, IL22, IL31, INS, KLK3, LGALS7, LHB, LYVE1, MICA, MICB, MMP10, MMP2, MMP7, MMP8, NCAM1, NID1, NRCAM, NRG1, PF4, PI3, PRSS27, RETN, SAA1, SCARB2, SERPINE1, SIGLEC9, SPP1, THRB, TNFRSF10B, TNFRSF11A, TNFRSF8, TREM1, TSLP, VCAM1, VEGFC |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adipsin (Complement Factor D)
| BCAM
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
| Fc gamma RIIB/C (CD32B/C)
|
Ferritin
| FLRG
| Follistatin
| Furin
| Galectin-7
|
GDF-15
| Growth Hormone
| IL-10 R alpha
| IL-22
| IL-28A (IFN-lambda 2)
|
IL-29 (IFN-lambda 1)
| IL-31
| Insulin
| Luteinizing hormone
| LIMPII
|
LYVE-1
| Marapsin
| MICA
| MICB
| MMP-2
|
MMP-7
| MMP-8
| MMP-10
| NCAM-1 (CD56)
| Nidogen-1
|
NrCAM
| NRG1-beta 1 (HRG1-beta 1)
| Osteopontin (SPP1)
| PAI-1
| Platelet factor 4 (CXCL4)
|
PSA-total
| RAGE
| RANK (TNFRSF11A)
| Resistin
| SAA (Serum Amyloid A)
|
Siglec-9
| TACE
| TIM-1 (KIM-1)
| TRAIL R2 (TNFRSF10B/DR5)
| Trappin-2
|
TREM-1
| TSH
| TSLP
| VCAM-1 (CD106)
| VEGF-C
|
XEDAR
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C9 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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