Human Cytokine Array (60 targets) (SARB0041)
- SKU:
- SARB0041
- Product Type:
- Protein Array
- Analytes:
- Angiogenin
- Analytes:
- BDNF
- Analytes:
- BLC (CXCL13)
- Analytes:
- BMP-4
- Analytes:
- BMP-6
- Analytes:
- Ck beta 8-1 (CCL23)
- Analytes:
- CNTF
- Analytes:
- EGF
- Analytes:
- Eotaxin-1 (CCL11)
- Analytes:
- Eotaxin-2 (MPIF-2/CCL24)
- Analytes:
- Eotaxin-3 (CCL26)
- Analytes:
- FGF-6
- Analytes:
- FGF-7 (KGF)
- Analytes:
- Flt-3 Ligand
- Analytes:
- Fractalkine (CX3CL1)
- Analytes:
- GCP-2 (CXCL6)
- Analytes:
- GDNF
- Analytes:
- GM-CSF
- Analytes:
- I-309 (TCA-3/CCL1)
- Analytes:
- IFN-gamma
- Analytes:
- IGFBP-1
- Analytes:
- IGFBP-2
- Analytes:
- IGFBP-4
- Analytes:
- IGF-1
- Analytes:
- IL-10
- Analytes:
- IL-13
- Analytes:
- IL-15
- Analytes:
- IL-16
- Analytes:
- IL-1 alpha (IL-1 F1)
- Analytes:
- IL-1 beta (IL-1 F2)
- Analytes:
- IL-1 ra (IL-1 F3)
- Analytes:
- IL-2
- Analytes:
- IL-3
- Analytes:
- IL-4
- Analytes:
- IL-5
- Analytes:
- IL-6
- Analytes:
- IL-7
- Analytes:
- Leptin
- Analytes:
- Light (TNFSF14)
- Analytes:
- MCP-1 (CCL2)
- Analytes:
- MCP-2 (CCL8)
- Analytes:
- MCP-3 (MARC/CCL7)
- Analytes:
- MCP-4 (CCL13)
- Analytes:
- M-CSF
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0041 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 60 |
| Gene Symbols: | ANG, BDNF, BMP4, BMP6, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18|CCL2, CCL20, CCL22, CCL23, CCL24, CCL26, CCL5, CCL7, CCL8, CNTF, CSF1, CSF2, CX3CL1, CXCL12, CXCL13, CXCL6, CXCL9, EGF, FGF6, FGF7, FLT3LG, GDNF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP4, IL10, IL13, IL15, IL16, IL1A, IL1B, IL1RN, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LTA, NTF3, PDGFB, PPBP, TGFB1, TGFB3, TNF, TNFSF14 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| BMP-4
| BMP-6
|
Ck beta 8-1 (CCL23)
| CNTF
| EGF
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-6
| FGF-7 (KGF)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GDNF
| GM-CSF
| I-309 (TCA-3/CCL1)
| IFN-gamma
|
IGFBP-1
| IGFBP-2
| IGFBP-4
| IGF-1
| IL-10
|
IL-13
| IL-15
| IL-16
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 ra (IL-1 F3)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| Leptin
| Light (TNFSF14)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
PARC (CCL18)
| PDGF-BB
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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