Name: Human Inflammation Array (20 targets) (SARB0050)
SKU: SARB0050
Availability: OutOfStock

Product Sku:	SARB0050
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	CCL1, CCL11, CCL24, CSF2, CSF3, CXCL8, IFNG, IL10, IL11, IL12A\|IL13, IL1A, IL1B, IL2, IL3, IL4, IL6, IL7, TIMP2
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579
IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301	IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809
IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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