Human Obesity Array (62 targets) (SARB0015)
- SKU:
- SARB0015
- Product Type:
- Protein Array
- Analytes:
- 4-1BB (TNFRSF9/CD137)
- Analytes:
- ACE-2
- Analytes:
- Adiponectin (ACRP30)
- Analytes:
- Adipsin (Complement Factor D)
- Analytes:
- AgRP
- Analytes:
- Angiopoietin-1
- Analytes:
- Angiopoietin-2
- Analytes:
- ANGPTL4
- Analytes:
- CRP (C-Reactive Protein)
- Analytes:
- ENA-78 (CXCL5)
- Analytes:
- Fas (TNFRSF6/Apo-1)
- Analytes:
- FGF-6
- Analytes:
- Growth Hormone
- Analytes:
- HCC-4 (CCL16)
- Analytes:
- IFN-gamma
- Analytes:
- IGFBP-1
- Analytes:
- IGFBP-2
- Analytes:
- IGFBP-3
- Analytes:
- IGF-1
- Analytes:
- IGF-1 R
- Analytes:
- IL-1 R4 (ST2)
- Analytes:
- IL-1 R1
- Analytes:
- IL-10
- Analytes:
- IL-11
- Analytes:
- IL-12 p70
- Analytes:
- IL-1 alpha (IL-1 F1)
- Analytes:
- IL-1 beta (IL-1 F2)
- Analytes:
- IL-6
- Analytes:
- IL-6 R
- Analytes:
- IL-8 (CXCL8)
- Analytes:
- Insulin
- Analytes:
- IP-10 (CXCL10)
- Analytes:
- Leptin R
- Analytes:
- Leptin
- Analytes:
- LIF
- Analytes:
- Lymphotactin (XCL1)
- Analytes:
- MCP-1 (CCL2)
- Analytes:
- MCP-3 (MARC/CCL7)
- Analytes:
- M-CSF
- Analytes:
- MIF
- Analytes:
- MIP-1 beta (CCL4)
- Analytes:
- MSP alpha/beta
- Analytes:
- Osteoprotegerin (TNFRSF11B)
- Analytes:
- Oncostatin M
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0015 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 62 |
| Gene Symbols: | ACE2, ADIPOQ, AGRP, ANGPT1, ANGPT2, ANGPTL4, CCL16, CCL18, CCL2, CCL25|CCL4, CCL5, CCL7, CFD, CRP, CSF1, CXCL10, CXCL12, CXCL5, CXCL8, EDA2R, FAS, FGF6, GH1, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL10, IL11, IL12A, IL1A, IL1B, IL1R1, IL1RL1, IL6, IL6R, INS, LEP, LEPR, LIF, MIF, MST1, OSM, PDGFA, PDGFB, RETN, SAA1, SERPINE1, TGFB1, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF9, TNFSF9, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Targets Included | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137) | ACE-2 | Adiponectin (ACRP30) | Adipsin (Complement Factor D) | AgRP |
| Angiopoietin-1 | Angiopoietin-2 | ANGPTL4 | CRP (C-Reactive Protein) | ENA-78 (CXCL5) |
| Fas (TNFRSF6/Apo-1) | FGF-6 | Growth Hormone | HCC-4 (CCL16) | IFN-gamma |
| IGFBP-1 | IGFBP-2 | IGFBP-3 | IGF-1 | IGF-1 R |
| IL-1 R4 (ST2) | IL-1 R1 | IL-10 | IL-11 | IL-12 p70 |
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2) | IL-6 | IL-6 R | IL-8 (CXCL8) |
| Insulin | IP-10 (CXCL10) | Leptin R | Leptin | LIF |
| Lymphotactin (XCL1) | MCP-1 (CCL2) | MCP-3 (MARC/CCL7) | M-CSF | MIF |
| MIP-1 beta (CCL4) | MSP alpha/beta | Osteoprotegerin (TNFRSF11B) | Oncostatin M | PAI-1 |
| PARC (CCL18) | PDGF-AA | PDGF-AB | PDGF-BB | RANTES (CCL5) |
| Resistin | SAA (Serum Amyloid A) | SDF-1 alpha (CXCL12 alpha) | TNF RII (TNFRSF1B) | TNF RI (TNFRSF1A) |
| TECK (CCL25) | TGF beta 1 | TIMP-1 | TIMP-2 | TNF alpha |
| VEGF-A | XEDAR | |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Obesity Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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