ELISpot

Human Perforin ELISpot Kit

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SKU:
HUDC0159
Product Type:
ELISpot
Reactivity:
Human

Description

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Human Perforin ELISpot Kit

Assay Genie ELISpot is a highly specific immunoassay for the analysis of Perforin production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of Perforin producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted Perforin (qualitative analysis) and single cells that produce Perforin (quantitative analysis). Cell secreted Perforin is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured Perforin is revealed by tracer antibodies and appropriate conjugates.

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