Human Phospho-P27/Kip1 (T198) and Total P27/Kip1 PharmaGenie ELISA Kit (SBRS1914)

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SKU:
SBRS1914
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phospho-P27/Kip1 (T198) and Total P27/Kip1 PharmaGenie ELISA Kit (SBRS1914)

Assay Genie Phospho- P27/Kip1 (T198) and Total P27/Kip1 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated P27/Kip1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho- P27/Kip1 and total P27/Kip1. An anti-pan P27/Kip1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and P27/Kip1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-P27/Kip1 (T198) antibody is used to detect phosphorylated P27/Kip1 or biotinylated anti-P27/Kip1 antibody is used to detect pan P27/Kip1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P27/Kip1 (T198) or pan P27/Kip1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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