Name: Human T Cell Response Array (20 targets) (SARB0068)
SKU: SARB0068
Availability: OutOfStock

Product Sku:	SARB0068
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	IFNG, IL10, IL12A, IL13, IL17A, IL17F, IL18, IL2, IL21, IL22\|IL23A, IL27, IL33, IL4, IL5, IL6, IL7, IL9, TGFB1, TNF
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
IL-2 Gene Symbol: IL2 IL-2 Gene ID: 3558 Accession #: P60568	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301	IL-12 p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137
TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552	IL-9 Gene Symbol: IL9 Gene ID: 3578 Accession #: P15248	IL-22 Gene Symbol: IL22 ILTIF ZCYTO18 UNQ3099/PRO10096 Gene ID: 50616 Accession #: Q9GZX6	IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4
IL-18 Gene Symbol: IL18 IGIF IL1F4 Gene ID: 3606 Accession #: Q14116	IL-23 p19 Gene Symbol: IL23A SGRF UNQ2498/PRO5798 Gene ID: 51561 Accession #: Q9NPF7	IL-21 Gene Symbol: IL21 Gene ID: 59067 Accession #: Q9HBE4	IL-27 Gene Symbol: IL27 IL27A IL30 Gene ID: 246778 Accession #: Q8NEV9	IL-33 (IL-1 F11) Gene Symbol: IL33 C9orf26 IL1F11 NFHEV Gene ID: 90865 Accession #: O95760

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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