Human TGF beta Array (25 targets) (SARB0070)
- SKU:
- SARB0070
- Product Type:
- Protein Array
- Analytes:
- Activin A
- Analytes:
- Activin R1A (ALK-2)
- Analytes:
- BAMBI (NMA)
- Analytes:
- beta IG-H3
- Analytes:
- BMP-2
- Analytes:
- BMP-4
- Analytes:
- BMP-6
- Analytes:
- BMP-7
- Analytes:
- BMP-8
- Analytes:
- BMP-9
- Analytes:
- BMP-15 (GDF-9B)
- Analytes:
- BMPR-IA (ALK-3)
- Analytes:
- BMPR-II
- Analytes:
- Follistatin
- Analytes:
- GDF-11 (BMP-11)
- Analytes:
- GDF-15
- Analytes:
- Lefty-A
- Analytes:
- Noggin
- Analytes:
- SMAD3
- Analytes:
- SMAD4
- Analytes:
- TGF alpha
- Analytes:
- TGF beta 1
- Analytes:
- TGF beta 2
- Analytes:
- TGF-beta RII
- Analytes:
- TGF beta R3
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0070 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 25 |
| Gene Symbols: | ACVR1, BAMBI, BMP15, BMP2, BMP4, BMP6, BMP7, BMP8A, BMPR1A, BMPR2|FST, GDF11, GDF15, GDF2, INHBA, LEFTY2, MADH3, NOG, SMAD4, TGFA, TGFB1, TGFB2, TGFBI, TGFBR2, TGFBR3 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Activin A
| Activin R1A (ALK-2)
| BAMBI (NMA)
| beta IG-H3
| BMP-2
|
BMP-4
| BMP-6
| BMP-7
| BMP-8
| BMP-9
|
BMP-15 (GDF-9B)
| BMPR-IA (ALK-3)
| BMPR-II
| Follistatin
| GDF-11 (BMP-11)
|
GDF-15
| Lefty-A
| Noggin
| SMAD3
| SMAD4
|
TGF alpha
| TGF beta 1
| TGF beta 2
| TGF-beta RII
| TGF beta R3
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human TGF beta Array C2 Membranes
- Blocking Buffer
- Biotinylated Antibody Cocktail
- 1,000X HRP-Streptavidin Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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