Metabolism Assays

Maltose Assay Kit (Colorimetric or Fluorometric) (BA0132)

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SKU:
BA0132
Product Type:
Assay
Detection Method:
Colorimetric
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Serum, Urine, Food, Beverages
Research Area:
TCA Cycle
Research Area:
Diabetes & Obesity
Research Area:
Food Safety & Analysis

Description

ELISA Kit Technical ManualMSDS

Maltose Assay Kit - Information

MALTOSE (C12H22O11) is a disaccharide, composed of two glucose units linked by an alpha bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products.

Assay Genie's maltose assay provides a simple, one step assay for measuring maltose. In this assay, maltose is converted to two glucoses, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at Ex/Em = 530/585 nm is directly proportional to maltose concentration in the sample.

Applications

Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.

Maltose Assay Kit - Key Features

  • Fast and sensitive. Use of 10 uL sample. Linear detection range 2 to 500 uM maltose in for colorimetric assays and 1 to 50 uM for flourimetric assays.
  • Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
  • High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Maltose Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL, Dye Reagent: 120 uL, Enzyme A: 120 uL, Standard: 1 mL, 5 mM Maltose Enzyme Mix: 120 uL
Kit Requires: Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader
Method of Detection: OD570nm, or FL530/585nm
Detection Limit: 2 uM
Samples: Serum, urine, food and beverages, etc.
Species: All
Protocol Length: 60 min
Size: 100 tests
Storage: Store all components at -20°C upon receiving
Shelf Life: 6 months

 

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