Monkey ELISA Kits
Monkey APOC3 / Apolipoprotein C-III ELISA Kit
- SKU:
- MKFI00008
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P18659
- Sensitivity:
- 0.938ng/ml
- Range:
- 1.563-100ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- ApoC3, Apolipoprotein C3, APOC3, ApoC-III, MGC150353, Apo-CIII, APOCIII, apoC-III
- Reactivity:
- Monkey
Description
Overview
Monkey APOC3 / Apolipoprotein C-III ELISA Kit
Apolipoprotein CIII (APOC3) is a vital protein involved in regulating lipid metabolism. It plays a crucial role in modulating the breakdown and clearance of triglyceride-rich lipoproteins, such as very-low-density lipoproteins (VLDL) and chylomicrons. This highly sensitive ELISA kit enables accurate quantification of APOC3, providing valuable insights into lipid disorders and metabolic syndrome in monkeys.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Monkey ApoC3 (Apolipoprotein C3) ELISA Kit |
| Product Code: | MKFI00008 |
| Size: | 96 Assays |
| Alias: | ApoC3, Apolipoprotein C3, APOC3, ApoC-III, MGC150353, Apo-CIII, APOCIII, apoC-III |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Target: | Monkey ApoC3 |
| Reactivity: | Monkey |
| Sensitivity: | 0.938ng/ml |
| Range: | 1.563-100ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Monkey ApoC3 and the recovery rates were calculated by comparing the measured value to the expected amount of Monkey ApoC3 in samples. Please contact us for more information. |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Monkey ApoC3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Please contact us for more information. |
| CV(%) | Intra-assay <8 Inter-assay <10 |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: Protocols are specific to each batch/lot. For the exact instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
| 6. | Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2. |
| 7. | Add 0.1 ml of Biotin- detecti Related Products     Human APOC3 / Apolipoprotein C-III ELISA Kit
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