Mouse Cell Signalling ELISA Kits 5

Mouse CD68 / Macrosialin ELISA Kit

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SKU:
MOFI00564
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31996
Sensitivity:
0.469ng/ml
Range:
0.781-50ng/ml
ELISA Type:
Sandwich
Synonyms:
CD68, Gp110, LAMP4, SCARD1, SCARD1, SR-D1, CD68 antigenmacrophage antigen CD68, molecule, macrosialin, SCARD1, scavenger receptor class D, member 1
Reactivity:
Mouse

Description

Mouse CD68 / Macrosialin ELISA Kit

CD68, also known as macrosialin, is a glycoprotein primarily expressed in cells of the monocyte/macrophage lineage. It is a transmembrane protein found in the lysosomes of these cells and is involved in various cellular processes. The Mouse Macrosialin ELISA Kit is designed to quantitatively measure the concentration of mouse macrosialin (CD68) in biological samples. It allows researchers to study the levels of this glycoprotein in various tissues and cell types, providing valuable insights into macrophage activation, immune responses, and inflammatory processes.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse CD68 / Macrosialin ELISA Kit

Product Code:

MOFI00564

Size:

96 Assays

Alias:

CD68, Gp110, LAMP4, SCARD1, SCARD1, SR-D1, CD68 antigenmacrophage antigen CD68, molecule, macrosialin, SCARD1, scavenger receptor class D, member 1

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse CD68 concentrations in serum plasma and other biological fluids.

Sensitivity:

0.469ng/ml

Range:

0.781-50ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse CD68 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse CD68 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

86-99

95

EDTA plasma (n=5)

88-100

93

UFH plasma (n=5)

85-99

91

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse CD68 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

91-105%

87-103%

87-97%

EDTA plasma (n=5)

85-97%

93-101%

8-95%

UFH Plasma (n=5)

80-99%

80-99%

82-100%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

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