Mouse Immunology ELISA Kits

Mouse Complement C4 / C4 ELISA Kit

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SKU:
MOFI00678
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01029
Sensitivity:
0.938ng/ml
Range:
1.563-100ng/ml
ELISA Type:
Sandwich
Synonyms:
C4
Reactivity:
Mouse
Research Area:
Immunology

Description

Mouse Complement C4 / C4 ELISA Kit

Complement C4, a pivotal protein within the complement system, plays a pivotal role in immune response modulation, opsonization, and clearance of immune complexes. The Mouse Complement C4 ELISA Kit not only facilitates the precise quantification of Complement C4 levels but also offers invaluable insights into its dynamic interactions, empowering researchers to gain profound insights into the complex realm of immune system orchestration.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse Complement C4 / C4 ELISA Kit

Product Code:

MOFI00678

Size:

96 Assays

Alias:

C4

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse C4 concentrations in serum plasma and other biological fluids.

Sensitivity:

0.938ng/ml

Range:

1.563-100ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse C4 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse C4 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

88-103

98

EDTA plasma (n=5)

87-105

95

UFH plasma (n=5)

85-97

91

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

87-101%

85-103%

87-91%

EDTA plasma (n=5)

83-99%

83-96%

88-93%

UFH Plasma (n=5)

82-100%

80-99%

80-92%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

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