Signaling Pathway Assays

pNPP Phosphatase Assay Kit (BA0167)

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SKU:
BA0167
Product Type:
Assay
Research Area:
Metabolism

Description

ELISA Kit Technical ManualMSDS

pNPP Phosphatase Assay Kit:

p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.

This homogeneous colorimetric "mix-and-measure" pNPP Phosphatase assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.

Applications

For quantitative determination of phosphatase activity.

pNPP Phosphatase Assay Kit - Key Features

  • High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below.
  • Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
  • Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

pNPP Phosphatase Assay Kit Information:

Kit Includes: Size (assays) 500, Reagent 280 mL, Assay Buffer 25 mL, Stop Solution 25 mL.  pNPP-HTS >10k customized.
Method of Detection: OD405nm
Detection Limit: 3 ng
Samples: Protein phosphatases
Species: All
Protocol Length: 30 min
Size: 500 tests
Storage: Store Reagent at -20°C and other components at 4°C.
Shelf Life: 12 months
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