Name: Rat Neuro Discovery Array (19 targets) (SARB0105)
SKU: SARB0105
Availability: OutOfStock

Product Sku:	SARB0105
Size:	2, 4, or 8
Species Detected:	Rat
Number of Targets Detected:	19
Gene Symbols:	BDNF, CCL11, CCL2, CNTF, CSF2, CXCL5, IFNG, IL10, IL1A, IL1B\|IL4, IL6, MOK, NGF, NOTCH1, NOTCH2, NRP1, TNF, VEGFA
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
beta-NGF Gene Symbol: NGF NGFB Gene ID: Accession #: P25427	CNTF Gene Symbol: CNTF Gene ID: 25707 Accession #: P20294	Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 29397 Accession #: P97545	GM-CSF Gene Symbol:CSF2 Gene ID: 116630 Accession #: P48750	IFN-gamma Gene Symbol: IFNG Gene ID: 25712 Accession #: P01581
IL-10 Gene Symbol: IL10 Gene ID: 25325 Accession #: P29456	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 24493 Accession #: P16598	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: Accession #: Q63264	IL-4 Gene Symbol: IL4 Gene ID: 287287 Accession #: P20096	IL-6 Gene Symbol: IL6 Gene ID: 24498 Accession #: P20607
LIX Gene Symbol: Cxcl5 Scyb5 Gene ID: 60665 Accession #: P97885	MCP-1 (CCL2) Gene Symbol: CCL2 SCYA2 Gene ID: 24770 Accession #: P14844	Neuropilin-1 Gene Symbol: NRP1 NRP VEGF165R Gene ID: 246331 Accession #: Q9QWJ9	Notch-1 Gene Symbol: NOTCH1 TAN1 Gene ID: 25496 Accession #: Q07008	Notch-2 Gene Symbol: Notch2 Gene ID: 29492 Accession #: Q9QW30
RAGE Gene Symbol: N/A Gene ID: Accession #: Q63495	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 103694380; 24835 Accession #: P16599	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 83785 Accession #: P16612	BDNF Gene Symbol: BDNF Gene ID: 24225 Accession #: P23363

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

इंटरल्यूकिन-8 सिग्नलिंग

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