Signaling Pathway Assays

TBARS Assay Kit (BA0060)

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SKU:
BA0060
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Saliva
Research Area:
Lipid Metabolism
Research Area:
Oxidative Stress
Research Area:
Lipid Peroxidation

Description

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TBARS Assay Kit - Information

Assay Genie's TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 560nm/585nm) is directly proportional to TBARS concentration in the sample.

Applications

For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.

TBARS Assay Kit - Key Features

Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 , fluorometric assay 0.1 - 1.5 MDA.

TBARS Assay Kit - Data Sheet

Kit Includes TBA Reagent: 25 mL Standard: 6 M MDA 10% Trichloroacetic acid (TCA): 25 mL
Kit Requires Pipetting devices, centrifuge tubes, centrifuge, clear flat-bottom uncoated 96-well plates, optical density or fluorescence plate readers, sonicator, water-bath or heat block.
Method of Detection OD535nm, or FL560/585nm
Detection Limit 0.1
Samples Serum, plasma, urine, saliva etc
Species All
Protocol Length 80 min
Size 100 tests
Storage Store all components at -20 °C
Shelf Life 6 months

More Details

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery.

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