ZEB2 Transcription Factor Activity Assay
- SKU:
- TFAB00206
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- ZEB2
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
Description
| Product Name: | ZEB2 Transcription Factor Activity Assay |
| Product Code: | TFAB00206 |
| Target: | ZEB2 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ZEB2 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; DNA-binding; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 2q22.3 Cellular Component: nucleus Molecular Function:protein binding Biological Process: negative regulation of transcription from RNA polymerase II promoter; pigmentation during development; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter Disease: Mowat-wilson Syndrome |
| NCBI Summary: | The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Code: | O60315 |
| NCBI GenInfo Identifier: | 13124503 |
| NCBI Gene ID: | 9839 |
| NCBI Accession: | O60315.1 |
| UniProt Secondary Accession: | O60315,Q9UED1, A0JP09, B7Z2P2, F5H814, |
| UniProt Related Accession: | O60315 |
| Molecular Weight: | 133,806 Da |
| NCBI Full Name: | Zinc finger E-box-binding homeobox 2 |
| NCBI Synonym Full Names: | zinc finger E-box binding homeobox 2 |
| NCBI Official Symbol: | ZEB2 |
| NCBI Official Synonym Symbols: | SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1 |
| NCBI Protein Information: | zinc finger E-box-binding homeobox 2 |
| UniProt Protein Name: | Zinc finger E-box-binding homeobox 2 |
| UniProt Synonym Protein Names: | Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b |
| Protein Family: | Zinc finger E-box-binding homeobox |
| UniProt Gene Name: | ZEB2 |
| UniProt Entry Name: | ZEB2_HUMAN |
