ELISpot

Human IL-5 ELISpot Pair

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SKU:
HUDC0173
Product Type:
ELISpot
Reactivity:
Human

Description

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Human IL-5 ELISpot Pair

Assay Genie ELISpot is a highly specific immunoassay for the analysis of IL-5 production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of IL-5 producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted IL-5 (qualitative analysis) and single cells that produce IL-5 (quantitative analysis). Cell secreted IL-5 is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured IL-5 is revealed by tracer antibodies and appropriate conjugates.

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