ELISpot

Human IL-6 ELISpot Pair

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SKU:
HUDC0174
Product Type:
ELISpot
Reactivity:
Human

Description

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Human IL-6 ELISpot Pair

Assay Genie ELISpot is a highly specific immunoassay for the analysis of IL-6 production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of IL-6 producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted IL-6 (qualitative analysis) and single cells that produce IL-6 (quantitative analysis). Cell secreted IL-6 is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured IL-6 is revealed by tracer antibodies and appropriate conjugates.

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