Name: Human Insulin Receptor Pathway Phosphorylation Array (9 targets) (SARB0053)
SKU: SARB0053
Availability: OutOfStock

Product Sku:	SARB0053
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	9
Gene Symbols:	EIF4E, FOXO3, IGF1R, INPP5D, INS, IRS1, PTPN11, SHC1, STK11
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	Akt Signaling / IGF Signaling / mTOR Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
eIF-4E (P-Ser209) Gene Symbol: EIF4E EIF4EL1 EIF4F Gene ID: 1977 Accession #: P06730	FOXO-3 (P-Ser413) Gene Symbol: FOXO3 Gene ID: 2309 Accession #: O43524	IGF-1 R (P-Tyr1165) Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069	Insulin (P-Tyr1189) Gene Symbol:INS Gene ID: 3630 Accession #: P01308	IRS-1 (P-Ser318) Gene Symbol: IRS1 Gene ID: 3667 Accession #: P35568
LKB1 (P-Ser428) Gene Symbol: STK11 LKB1 PJS Gene ID: 6794 Accession #: Q15831	SHC (P-Tyr427) Gene Symbol: SHC1 SHC SHCA Gene ID: 6464 Accession #: P29353	SHIP1 (P-Tyr1020) Gene Symbol: INPP5D SHIP SHIP1 Gene ID: 3635 Accession #: Q92835	SHP-2 (P-Ser542) Gene Symbol: PTPN11 PTP2C SHPTP2 Gene ID: 5781 Accession #: Q06124

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated anti-IgG
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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