Name: Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)
SKU: SARB0059
Availability: OutOfStock

Product Sku:	SARB0059
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	11
Gene Symbols:	ATM, EIF2S1, HDAC2, HDAC4, MAP3K7, NFKBIA, RELA, RPS6KA5, STAT1, TBK1\|ZAP70
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PKC Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472; Accession #: Q13315	eIF-2a (P-Ser52) Gene Symbol: EIF2S1 EIF2A Gene ID: 1965; Accession #: P05198	HDAC2 (P-Ser394) Gene Symbol: HDAC2 Gene ID: 3066 Accession #: Q92769	HDAC4 (P-Ser632) Gene Symbol: HDAC4 KIAA0288 Gene ID: 9759; Accession #: P56524	IKB-alpha (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792; Accession #: P25963
MSK1 (P-Ser376) Gene Symbol: RPS6KA5 MSK1 Gene ID: 9252; Accession #: O75582	NF-kB (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970; Accession #: Q04206	STAT1 (P-Ser727) Gene Symbol: STAT1 Gene ID: 6772; Accession #: P42224	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885; Accession #: O43318	TBK1 (P-Ser172) Gene Symbol: TBK1 NAK Gene ID: 29110; Accession #: Q9UHD2
ZAP70 (P-Tyr292) Gene Symbol: ZAP70 SRK Gene ID: 7535; Accession #: P43403

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or plastic Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated anti-IgG
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

इंटरल्यूकिन-8 सिग्नलिंग

ग्लाइकोलाइसिस मार्ग विस्तार से: ग्लूकोज को ऊर्जा में कैसे बदला जाता है