Name: Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)
SKU: SARB0055
Availability: OutOfStock

Product Sku:	SARB0055
Size:	2, 4, or 8
Species Detected:	Human / Mouse
Number of Targets Detected:	17
Gene Symbols:	AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14\|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	Akt Signaling / HER/ErbB Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / PI3K-AKT Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749	CREB (P-Ser133) Gene Symbol: CREB1 Gene ID: 1385 Accession #: P16220	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5594 Accession #: P28482	GSK3a (P-Ser21) Gene Symbol: GSK3A Gene ID: 2931 Accession #: P49840	GSK3b (P-Ser9) Gene Symbol: GSK3B Gene ID: 2932 Accession #: P49841
HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792	JNK (P-Thr183) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983	MEK (P-Ser217/Ser221) Gene Symbol: MAP2K1 MEK1 PRKMK1 Gene ID: 5604 Accession #: Q02750	MKK3 (P-Ser189) Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Gene ID: 5606 Accession #: P46734	MKK6 (P-Ser207) Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3 Gene ID: 5608 Accession #: P52564
MSK2 (P-Ser360) Gene Symbol: RPS6KA4 MSK2 Gene ID: 8986 Accession #: O75676	mTOR (P-Ser2448) Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1 Gene ID: 2475 Accession #: P42345	p38 (P-Thr180/Tyr182) Gene Symbol: MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A Gene ID: 1432 Accession #: Q16539	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637	P70S6K (P-Thr421/Ser424) Gene Symbol: RPS6KB1 STK14A Gene ID: 6198 Accession #: P23443
RSK1 (P-Ser380) Gene Symbol: RPS6KA1 MAPKAPK1A RSK1 Gene ID: 6195 Accession #: Q15418	RSK2 (P-Ser386) Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2 Gene ID: 10432 Accession #: P51812

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated anti-IgG
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

इंटरल्यूकिन-8 सिग्नलिंग

ग्लाइकोलाइसिस मार्ग विस्तार से: ग्लूकोज को ऊर्जा में कैसे बदला जाता है