Name: Human RTK Phosphorylation Array (71 targets) (SARB0064)
SKU: SARB0064
Availability: OutOfStock

Product Sku:	SARB0064
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	71
Gene Symbols:	ABL1, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1\|EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, ERBB2, ERBB3, ERBB4, FER, FGFR1, FGFR2, FGR, FLT4, FRK, FYN, HCK, IGF1R, INSR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, LTK, LYN, MATK, MET, MUSK, NGFR, NTRK2, PDGFRA, PDGFRB, PTK2, PTK2B, RET, ROR1, ROR2, ROS1, RYK, SLPI, SRMS, SYK, TEC, TEK, TIE1, TNK1, TNK2, TXK, TYK2, TYRO3, ZAP70
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	PI3K-AKT Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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ABL1 Gene Symbol: ABL1 Gene ID: 25 Accession #: P00519	ACK Gene Symbol: TNK2 Gene ID: 10188 Accession #: Q07912	ALK-1 Gene Symbol: ALK Gene ID: 238 Accession #: Q9UM73	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530	Blk Gene Symbol: BLK Gene ID: 640 Accession #: P51451
BMX Gene Symbol: BMX Gene ID: 660 Accession #: P51813	Btk Gene Symbol: BTK Gene ID: 695 Accession #: Q06187	Csk Gene Symbol: CSK Gene ID: 1445 Accession #: P41240	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533
EphA1 Gene Symbol: EphA1 Gene ID: 2041 Accession #: P21709	EphA2 Gene Symbol: EphA2 Gene ID: 1969 Accession #: P29317	EphA3 Gene Symbol: EphA3 Gene ID: 2042 Accession #: P29320	EphA4 Gene Symbol: EphA4 Gene ID: 2043 Accession #: P54764	EphA5 Gene Symbol: EphA5 Gene ID: 2044 Accession #: P54756
EphA6 Gene Symbol: EphA6 Gene ID: 285220 Accession #: Q9UF33	EphA7 Gene Symbol: EphA7 Gene ID: 2045 Accession #: Q15375	EphA8 Gene Symbol: EphA8 Gene ID: 2046 Accession #: P29322	EphB1 Gene Symbol: EphB1 Gene ID: 2047 Accession #: P54762	EphB2 Gene Symbol: EphB2 Gene ID: 2048 Accession #: P29323
EphB3 Gene Symbol: EphB3 Gene ID: 2049 Accession #: P54753	EphB4 Gene Symbol: EphB4 Gene ID: 2050 Accession #: P54760	EphB6 Gene Symbol: EphB6 Gene ID: 2051 Accession #: O15197	ErbB2 Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB3 Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860
ErbB4 Gene Symbol: ErbB4 Gene ID: 2066 Accession #: Q15303	FAK Gene Symbol: PTK2 Gene ID: 5747 Accession #: Q05397	FER Gene Symbol: FER Gene ID: 2241 Accession #: P16591	FGFR1 Gene Symbol: FGFR1 Gene ID: 2260 Accession #: P11362	FGFR2 Gene Symbol: FGFR2 Gene ID: 2263 Accession #: P21802
FGFR2 alpha isoform Gene Symbol: FGFR2 Gene ID: 2263 Accession #: P21802	Fgr Gene Symbol: FGR Gene ID: 2268 Accession #: P09769	FRK Gene Symbol: FRK Gene ID: 2444 Accession #: P42685	Fyn Gene Symbol: FYN Gene ID: 2534 Accession #: P06241	Hck Gene Symbol: HCK Gene ID: 3055 Accession #: P08631
HGFR Gene Symbol: MET Gene ID: 4233 Accession #: P08581	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069	Insulin R (CD220) Gene Symbol: INSR Gene ID: 3643 Accession #: P06213	Itk Gene Symbol: ITK Gene ID: 3702 Accession #: Q08881	JAK1 Gene Symbol: JAK1 Gene ID: 3716 Accession #: P23458
JAK2 Gene Symbol: JAK2 Gene ID: 3717 Accession #: O60674	JAK3 Gene Symbol: JAK3 Gene ID: 3718 Accession #: P52333	LCK Gene Symbol: LCK Gene ID: 3932 Accession #: P06239	LTK Gene Symbol: LTK Gene ID: 4058 Accession #: P29376	Lyn Gene Symbol: LYN Gene ID: 4067 Accession #: P07948
MATK Gene Symbol: MATK Gene ID: 4145 Accession #: P42679	M-CSF R Gene Symbol: CSF1R Gene ID: 1436 Accession #: P07333	MUSK Gene Symbol: MuSK Gene ID: 4593 Accession #: O15146	NGFR (TNFRSF16) Gene Symbol: NGFR Gene ID: 4804 Accession #: P08138	PDGFRA Gene Symbol: PDGFRA Gene ID: 5156 Accession #: P16234
PDGFRB Gene Symbol: PDGFRB Gene ID: 5159 Accession #: P09619	PYK2 Gene Symbol: PTK2B Gene ID: 2185 Accession #: Q14289	RET Gene Symbol: RET Gene ID: 5979 Accession #: P07949	ROR1 Gene Symbol: ROR1 Gene ID: 4919 Accession #: Q01973	ROR2 Gene Symbol: ROR2 Gene ID: 4920 Accession #: Q01974
ROS Gene Symbol: ROS1 Gene ID: 6098 Accession #: P08922	RYK Gene Symbol: RYK Gene ID: 6259 Accession #: P34925	SCF R (CD117/c-kit) Gene Symbol: KIT Gene ID: 3815 Accession #: P10721	SRMS Gene Symbol: SRMS Gene ID: 6725 Accession #: Q9H3Y6	SYK Gene Symbol: SYK Gene ID: 6850 Accession #: P43405
Tec Gene Symbol: TEC Gene ID: 7006 Accession #: P42680	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763	TNK1 Gene Symbol: TNK1 Gene ID: 8711 Accession #: Q13470	TRKB Gene Symbol: NTRK2 Gene ID: 4915 Accession #: Q16620
TXK Gene Symbol: TXK Gene ID: 7294 Accession #: P42681	Tyk2 Gene Symbol: TYK2 Gene ID: 7297 Accession #: P29597	TYRO10 (DDR2/TKT) Gene Symbol: DDR2 Gene ID: 4921 Accession #: Q16832	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916
ZAP70 Gene Symbol: ZAP70 Gene ID: 7535 Accession #: P43403

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Human RTK Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Anti-Phospho Tyrosine Detection Antibody
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer (1 x 5 ml)
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail II
8-Well Incubation Tray
Plastic Sheets
Array Templates

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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Human RTK Phosphorylation Array (71 targets) (SARB0064)

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Human RTK Phosphorylation Array (71 targets) (SARB0064)

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Human RTK Phosphorylation Array (71 targets) (SARB0064)

Description

Overview

Target Information

Product Features

Kit Components

Other Materials Required

Protocol Outline

0 Reviews

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