Mouse Growth Factor Array (10 targets) (SARB0086)

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SKU:
SARB0086
Product Type:
Protein Array
Analytes:
bFGF
Analytes:
EGF
Analytes:
GCSF
Analytes:
GM-CSF
Analytes:
HGF
Analytes:
IGF-1
Analytes:
IGF-2
Analytes:
M-CSF
Analytes:
SCF
Analytes:
VEGF-A
Reactivity:
Mouse
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0086
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:10
Gene Symbols:CSF1, CSF2, CSF3, EGF, FGF2, HGF, IGF1, IGF2, KITLG, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / IGF Signaling / p53 Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 14173
  • Accession #: P15655
EGF
  • Gene Symbol: EGF
  • Gene ID: 13645
  • Accession #: P01132
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 12985
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 12983
  • Accession #: P26955
HGF
  • Gene Symbol: MET
  • Gene ID: 15234
  • Accession #: Q08048
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 16000
  • Accession #: P05017
IGF-2
  • Gene Symbol: IGF2 PP1446
  • Gene ID: 16002
  • Accession #: P09535
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 12977
  • Accession #: P07141
SCF
  • Gene Symbol: KITLG SCF
  • Gene ID: 17311
  • Accession #: P20826
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 22339
  • Accession #: Q00731
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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