Name: Mouse Growth Factor Array (20 targets) (SARB0087)
SKU: SARB0087
Availability: OutOfStock

Product Sku:	SARB0087
Size:	2, 4, or 8
Species Detected:	Mouse
Number of Targets Detected:	20
Gene Symbols:	AREG, CSF1, CSF2, CSF3, EGF, FGF2, FIGF, FLT1, FLT4, HGF\|IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL2, IL7, KITLG, VEGFA
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	IGF Signaling / p53 Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Amphiregulin Gene Symbol: AREG SDGF; AREGB Gene ID: 11839 Accession #: P31955	bFGF Gene Symbol: FGF2 Gene ID: 14173 Accession #: P15655	EGF Gene Symbol: EGF Gene ID: 13645 Accession #: P01132	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 12985 Accession #: P09920	GM-CSF Gene Symbol: CSF2 Gene ID: 12983 Accession #: P26955
HGF Gene Symbol: MET Gene ID: 15234 Accession #: Q08048	IGF-1 Gene Symbol: IGF1 Gene ID: 16000 Accession #: P05017	IGF-2 Gene Symbol: IGF2 PP1446 Gene ID: 16002 Accession #: P09535	IGFBP-2 Gene Symbol: N/A Gene ID: 16008 Accession #: P47877	IGFBP-3 Gene Symbol: IGFBP3 IBP3 Gene ID: 16009 Accession #: P47878
IGFBP-5 Gene Symbol: IGFBP5 IBP5 Gene ID: 16011 Accession #: Q07079	IGFBP-6 Gene Symbol: IGFBP6 IBP6 Gene ID: 16012 Accession #: P47880	IL-2 Gene Symbol: IL2 IL-2 Gene ID: 16183 Accession #: P04351	IL-7 Gene Symbol: IL7 Gene ID: 16196 Accession #: P10168	M-CSF Gene Symbol: CSF1 Gene ID: 12977 Accession #: P07141
SCF Gene Symbol: KITLG SCF Gene ID: 17311 Accession #: P20826	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 22339 Accession #: Q00731	VEGF-D Gene Symbol: FIGF VEGFD Gene ID: 14205 Accession #: P97946	VEGFR1 Gene Symbol: FLT1 FLT FRT VEGFR1 Gene ID: 14254 Accession #: P35969	VEGFR3 Gene Symbol: FLT4 VEGFR3 Gene ID: 14257 Accession #: P35917

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

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