Rat AKI Array (7 targets) (SARB0099)
- SKU:
- SARB0099
- Product Type:
- Protein Array
- Analytes:
- TIM-1 (KIM-1)
- Analytes:
- FABP1
- Analytes:
- MCP-1 (CCL2)
- Analytes:
- Cystatin C
- Analytes:
- Lipocalin-2 (NGAL)
- Analytes:
- TIMP-1
- Analytes:
- VEGF-A
- Reactivity:
- Rat
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0099 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 7 |
| Gene Symbols: | CCL2, CST3, FABP1, HAVCR1, LCN2, TIMP1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
TIM-1 (KIM-1)
| FABP1
| MCP-1 (CCL2)
| Cystatin C
| Lipocalin-2 (NGAL)
|
TIMP-1
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Acute Kidney Injury (AKI) Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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